08-P021 PIP5K-dependent production of PIP2 sustains microtubule organization to establish polarized transport in the Drosophila oocyte

with ASPM siRNA, there is defective cleavage furrow orientation, cytokinesis failure and an increase in apoptosis. Dominant negative expression of GFP-ASPM C-terminal fusion proteins induces cytokinesis failure in HeLa cells. To further investigate the functions of ASPM in cell division we are currently performing a foetal brain yeast two-hybrid (Y2H) screen to identify direct interactants of the ASPM C-terminal domain. At present, the only reported interactant of the ASPM C-terminus is citron kinase, a protein with a known role in cytokinesis. We will discuss our findings of this association and the results of our Y2H screen.